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SDS-PAGE analysis of purification of Per a 5 in baculovirus-infected insect cells through Ni column. Lane M: protein standard; lane 1: elute with the elution buffer containing 250 mM imidazole; lane 2: elute with the elution buffer containing 20 mM imidazole; lane 3: elute with the elution buffer containing 50 mM imidazole; lane 4: run through; lane 5: the cytosol.
