Table 1.
PCR and RFLP procedures and products of NF-κB1 -94ins/delATTG and NF-κBIA 3′UTR A→G.
| Primers (forward and reverse) | PCR product | Restriction enzyme | Restriction products | |
|---|---|---|---|---|
| NF-κB1 | 5′-TGGGCACAAGTCGTTTATGA-3′ | 285 bp | Van91l (PflMI) | ATTG2/ATTG2 (ins/ins): 240 bp, 45 bp |
| 5′-CTGGAGCCGGTAGGGAAG-3′ | ATTG1/ATTG1 (del/del): 281 | |||
| ATTG2/ATTG1 (ins/del): 281, 240, 45 | ||||
| NF-κBIA | 5′-GGCTGAAAGAACATGGACTTG-3′ | 424 bp | HaeIII | AA wild type: 424 |
| 5′-GTACACCATTTACAGGAGGG-3′ | AG heterozygous: 316, 108 | |||
| GG mutant: 424, 316, 108 |