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. 2014 Mar 13;10(3):e1004001. doi: 10.1371/journal.ppat.1004001

Figure 7. Transmission of Nipah virus (strain Bangladesh/200401066) in Syrian hamsters.

Figure 7

Shedding of Nipah virus in inoculated (left panels) and naïve (right panels) animals in fomite, contact and aerosol transmission. Hamsters were inoculated intranasally with 107 TCID50 of Nipah virus (strain Bangladesh/200401066) or via drinking of 5×108 TCID50 of Nipah virus (strain Bangladesh/200401066) in artificial palm sap; nasal (white bars) and pharyngeal (black bars) swabs were collected daily. Seroconversion in naïve hamsters at 28 dpi as determined by ELISA is indicated on the right as the number of seroconverted hamsters/total number of exposed hamsters. Viral load in the swabs was determined as TCID50 equivalents by real-time RT-PCR. TCID50 equivalents were extrapolated from standard curves generated by adding dilutions of RNA extracted from a Nipah virus stock with a known virus titer in parallel to each run. Geometric mean viral loads are displayed; error bars indicate standard deviation.