Fig. 4.

Control experiments. A,B, Transgenic mouse lacking SynI or SynII proteins are used as negative controls. Genetic deletion of SynI or SynII proteins eliminated SynI (A) and SynII (B) labeling in cortical fibers, while VGluT2 stain was unaffected. C,D, Colocalization of synapsin and VGluT2 occurs in other brain areas. C, In hippocampal subiculum, VGluT2 and SynI were colocalized in a narrow band close to the medial border (Hc sub). PC analysis yielded high positive correlation within a band that was marked within two yellow stippled lines. Scale bar =10 μm (applies to all panels). D, VGluT2 and SynII were colocalized in a region apical to the pyramidal cells of hippocampal lucidum (Hc luc).