Table 1.

Purification of Recombinant Human Mitochondrial SSB

	Fraction	Volume (mL)	Protein (mg/mL)	Total Protein (mg)
I.	Lysate	19	13	250
II.	Affi-Gel Blue	52	0.40	21
III.	Ammonium Sulfate	2.1	6.7	14
IV.	MonoS	3.0	1.5	4.4
V.	MonoQ	5.8	0.74	4.3
VI.	Dialysed	3.6	1.2	4.3

The cDNA for mature mtSSB was expressed in E. coli, and the protein was purified to homogeneity as described in Subheading 3.1 to 3.7.