Table 1. Manual and automatic measurements on dataset.

Image	Manual a	Automatic b		Manual	Automatic		Manual	Automatic
#1	27±0.2	28	#35	31±0.2	31	#69	36±0.2	36
#2	20±1.2	21	#36	35±0.9	35	#70	35±0.2	35
#3	28±0.4	28	#37	40±0.6	39	#71	35±0.2	34
#4	34±0.3	34	#38	40±0.4	40	#72	25±0.3	25
#5	34±0.3	34	#39	31±2	32	#73	16±0.1	16
#6	33±0.1	32	#40	36±1.8	36	#74	28±0.8	27
#7	34±0.6	34	#41	34±0.9	34	#75	12±0.4	11
#8	14±1.1	13	#42	26±1	25	#76	14±0.4	14
#9	16±0.6	16	#43	31±0.6	30	#77	17±0.9	17
#10	22±2.7	22	#44	34±0.2	34	#78	19±0.4	19
#11	32±1.3	32	#45	18±1.7	17	#79	19±0.3	19
#12	30±1.6	29	#46	25±0.6	26	#80	20±0.6	20
#13	34±0.7	34	#47	28±0.5	27	#81	16±0.3	15
#14	28±2.3	28	#48	33±0.4	33	#82	16±0.3	16
#15	37±0.1	36	#49	32±0.2	32	#83	16±0.4	16
#16	23±0.2	22	#50	26±0.2	27	#84	17±0.6	17
#17	27±0.6	28	#51	36±0.8	36	#85	26±1.1	25
#18	28±0.6	29	#52	36±0.5	37	#86	27±0.5	26
#19	27±0.4	27	#53	37±0.3	37	#87	8±0.6	8
#20	30±0.6	30	#54	36±1.1	36	#88	8±0.3	7
#21	26±0.1	26	#55	31±0.3	32	#89	7±0.2	8
#22	32±0.1	33	#56	26±1.1	26	#90	9±0.6	9
#23	32±0.6	33	#57	26±1.6	26	#91	9±0.6	9
#24	34±1.4	34	#58	32±0.8	32	#92	11±0.4	11
#25	35±0.6	36	#59	37±0.3	38	#93	12±0.2	12
#26	12±0.5	12	#60	23±1.4	23	#94	12±0.2	12
#27	13±0.5	12	#61	24±0.2	24	#95	13±0.5	12
#28	29±0.9	29	#62	34±0.3	34	#96	36±0.3	36
#29	39±0.8	39	#63	29±0.7	30	#97	34±0.2	34
#30	39±0.5	39	#64	35±1	35	#98	33±0.7	33
#31	32±0.1	32	#65	32±0.4	33	#99	31±0.4	31
#32	32±0.3	32	#66	34±0.3	34	#100	32±0.4	32
#33	34±0.3	34	#67	32±0.1	33	#101	25±0.2	25
#34	22±0.3	23	#68	26±0.2	26	#102	31±0.4	30

^aAveraged values (mean ± SD) from measurements (in µm) taken by 3 different users.

^bAutomatically measured values (in µm) taken by the algorithm with default parameter values (i.e. level = 1, preferred smoothing spacing = 25, pixel tolerance = 1, and no additional processing).