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. 2014 Mar 13;9(3):e91723. doi: 10.1371/journal.pone.0091723

Figure 1. Purification of HrCHT1a and HrCHT1b.

Figure 1

(A) Silver stained 15% SDS-PAGE gel showing apoplastic proteins isolated from non-acclimated and 3 weeks cold acclimated seedlings. (B) SDS-PAGE and (C) 10% native gel showing purified class I chitinase from 3 weeks cold acclimated apoplastic extracts using chitin affinity chromatography. (D) Two dimensional gel electrophoresis of HrCHT1a and HrCHT1b (E). Purified HrCHT1a and HrCHT1b (70 µg) were loaded on 3–10 non-linear IPG strips and subjected to iso-electric focusing. After IEF, proteins were resolved on 15% SDS-PAGE for the second dimension.