Table 1. Quantitative analysis of CD spectra of HrCHT1a and HrCHT1b.

S. No	Protein/treatment	Percentage of α-helix	Percentage of β-strand
1	NA-HrCHT1a	4.51	36.47
2	NA-HrCHT1a+Ca	3.84	37.9
3	NA-HrCHT1a+EGTA	3.86	37.85
4	CA-HrCHT1a	2.37	39.39
5	CA-HrCHT1a+Ca	0.92	41.9
6	CA-HrCHT1a+EGTA	1.30	41.35
7	NA-HrCHT1b	2.85	40.37
8	NA-HrCHT1b+Ca	1.37	41.73
9	NA-HrCHT1b+EGTA	1.59	42.06
10	CA-HrCHT1b	1.08	43.55
11	CA-HrCHT1b+Ca	1.01	43.39
12	CA-HrCHT1b+EGTA	1.01	43.29

The percentage of α-helix and β-strand in HrCHT1a and HrCHT1b isolated from non-acclimated (NA) and cold acclimated (CA) seedlings were calculated using K2D3 server, in presence or absence of calcium.