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. 2014 Mar 13;9(3):e91782. doi: 10.1371/journal.pone.0091782

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© 2014 Xiao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Schematic representations of HA-tagged BEX1 deletion mutants and their ability to bind to BCL-2. B–D, HEK293 cells were transfected with plasmids expressing HA-BEX1 and HA-tagged BEX1 deletion mutants. BEX1 was immunoprecipitated using an anti-HA antibody, and the co-immunoprecipitate (co-IP) was analyzed by immunoblotting with an anti-BCL-2 antibody. BCL-2 was immunoprecipitated using an anti-BCL-2 antibody or isotype control rabbit IgG, and the co-IP was analyzed by IB with an anti-HA antibody. The presence of HA-BEX1, HA-tagged BEX1 deletion mutants and BCL-2 were monitored in the WCE.