Table 1. Identification of BCL-2 as an interaction partner for BEX1 by a yeast two-hybrid screen.
BD plasmid | AD plasmid | Growth on -Ade-His |
pGBKT7/BEX1 | – | – |
– | pGADT7-Rec/BCL-2 | – |
pGBKT7/BEX1 | pGADT7-Rec/BCL-2 | + |
pGBKT7/P53 | pGADT7-Rec/SV40-T | + |
pGBKT7/LAM | pGADT7-Rec/SV40-T | – |
BD, binding domain; AD, activation domain; LAM, laminin C; SV40-T, SV40 large T; P53, protein 53.