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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Mol Pharmacol. 2001 Nov;60(5):1057–1063.

Fig. 2.

Fig. 2

Effects of VUF5455 on the dissociation of [125I]I-AB-MECA from human A3 adenosine receptors expressed in HEK293 cells. [125I]I-AB-MECA (0.15 nM) was preassociated with the A3 adenosine receptor membranes for 1 h at 37°C in a total assay volume of 100 μl. Nonspecific binding was determined in parallel by addition of 30 μM NECA before the preassociation. After 1 h preassociation, dissociation was initiated by addition of NECA (final concentration, 30 μM) alone or with VUF5455. The incubations were terminated by filtration through GF/B filters on a cell harvester (Brandel) after additional times as indicated. The data shown were derived from one experiment performed in duplicate and are typical of three independent experiments giving similar results.