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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Mol Pharmacol. 2001 Nov;60(5):1057–1063.

Fig. 3.

Fig. 3

Time course of the dissociation of [3H]PSB-11 from the human A3 adenosine receptor. Membranes (60 μg) were preincubated with 4 nM [3H]PSB11 in a total assay volume of 100 μl for 120 min at 4°C. The dissociation of [3H]PSB-11 from the A3 adenosine receptor was started by addition of 30 μM NECA in the absence or presence of tested compounds. Nonspecific binding was measured after 120 min incubation in the presence of 30 μM NECA. The reaction was measured by rapid filtration at appropriate time intervals. Data are from one experiment performed in duplicate; two additional experiments gave similar results.