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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Mol Pharmacol. 2001 Nov;60(5):1057–1063.

Fig. 5.

Fig. 5

Competition of [125I]I-AB-MECA binding to human A3 adenosine receptors expressed in HEK293 cells by VUF5455 andVUF8504. Binding of 0.15 nM [125I]I-AB-MECA to membranes of HEK293 cells expressing human A3 adenosine receptors (25 μg of protein) was carried out in duplicate at 37°C for 1 h in 100 μl 50 mM Tris-HCl buffer, pH 8.0, containing 10 mM MgCl2, 1 mM EDTA, and 0.1 mg/ml CHAPS. The reaction was terminated by rapid filtration through GF/B filters by using a Brandel cell harvester. The figure shows representative experiments with VUF5455 and VUF8504.