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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Mol Pharmacol. 2001 Nov;60(5):1057–1063.

Fig. 8.

Fig. 8

Time course of the dissociation of [125I]I-AB-MECA dissociation from rat A3 adenosine receptors. [125I]I-AB-MECA (0.15 nM) was preassociated with the rat A3 adenosine receptor membranes (60 μg of protein) at 37°C for 1 h. Nonspecific binding was determined in parallel by addition of 30 μM NECA before the preassociation. After 1-h preassociation, dissociation was initiated by addition of NECA (final concentration, 30 μM) and tested agents. The incubation was terminated by filtration through GF/B filters on a Brandel cell harvester. The data shown were derived from one experiment performed in duplicate and are typical of three independent experiments giving similar results.