Figure 4.

Suppression of MDH aggregation by TbHsp70.c, TcHsp70B and Tbj2. The reaction was initiated by the addition of MDH (0.715 µM) to the assay buffer at 48°C; the concentrations of all components are given in the figure. TcHsp70B suppresses aggregation of MDH. TbHsp70.c and Tbj2 both suppress aggregation of MDH in a dose-dependent manner. The effect of Tbj2 on the ability of TbHsp70.c and TcHsp70B to suppress MDH aggregation was investigated. Each assay was conducted in triplicate and three independent experiments on independent batches of protein were conducted and the data shown represents that of a typical experiment. The bars represent standard deviations. *P < 0.001, **P < 0.01 and ***P < 0.05.