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. 2014 Mar 14;5:94. doi: 10.3389/fpls.2014.00094

Figure 2.

Figure 2

Thermospermine down-regulates the genes that are up-regulated in acl5-1. (A,B) Effect of exogenous thermospermine and spermine in acl5-1 (A) and wild-type (B) seedlings on the expression of the genes up-regulated in acl5-1. Transcript levels in seedlings grown for 7 days in MS solutions and incubated for 24 h in MS plus 100 μM thermospermine (black bars) and those in seedlings grown for 7 days in MS solutions supplemented with thermospermine (dark gray bars) or spermine (light gray bars) are shown relative to those in seedlings grown for 7 days in MS solutions with no polyamines (white bars). (C) Effect of endogenously-induced thermospermine on the expression of the genes up-regulated in acl5-1. Transcript levels in acl5-1 seedlings heat-shocked at 37°C for 2 h followed by 24-h culture at 22°C (light gray bars), those in transgenic acl5-1 seedlings carrying the HS-ACL5 construct untreated (dark gray bars) and those in transgenic acl5-1 seedlings carrying the HS-ACL5 construct heat-shocked at 37°C for 2 h followed by 24-h culture at 22°C (black bars), are shown relative to those in acl5-1 seedlings untreated (white bars). Error bars represent the SE (n = 3).