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. 2014 Mar 14;5:94. doi: 10.3389/fpls.2014.00094

Figure 6.

Figure 6

Responses of SAC51 to polyamines in acl5-1. (A) Effect of endogenously-induced thermospermine on SAC51 expression. Transcript levels in acl5-1 seedlings heat-shocked at 37°C for 2 h followed by 24-h culture at 22°C (light gray bars), those in transgenic acl5-1 seedlings carrying the HS-ACL5 construct untreated (dark gray bars) and those heat-shocked at 37°C for 2 h followed by 24-h culture at 22°C (black bars), are shown relative to those in acl5-1 seedlings untreated (white bars). (B) Effect of polyamines on SAC51-GUS expression. Transgenic acl5-1 seedlings carrying the GUS gene fused to the SAC51 promoter and its 5′ leader sequence were grown for 7 days in MS solutions supplemented with each polyamine. Numbers correspond to those of the carbon shown in Table 1. The activity is shown relative to that of mock-treated seedlings. Error bars represent the SE (n = 3).