Table 1.

Primer sets for amplification of carbapenem resistance determine genes (16).

Gene	Primer sequence (5′→ 3′)	TM (°C)	Amplicons size (bp)
bla-VIM	Forward: GATGGTGTTTGGTCGCATA Reverse: CGAATGCGCAGCACCAG	54.5  57.6	390
bla-KPC	Forward: CATTCAAGGGCTTTCTTGCTGC Reverse: ACGACGGCATAGTCATTTGC	60.3  57.3	498
bla-NDM	Forward: GGTTTGGCGATCTGGTTTTC Reverse: CGGAATGGCTCATCACGATC	57.3  59.4	521
bla-IMP-1	Forward: TTGACACTCCATTTACAG Reverse: GATTGAGAATTAAGCCACTCT	49.1  54.0	232
bla-IMP-2	Forward: TTGACACTCCATTTACGG Reverse: GATCGAGAATTAAGCCACCCT	51.4  57.9	232
bla-IMP-3	TTGACACTCCATTTACTG GATCGAGAATTAAGCCACTCT	49.1  55.9	232
bla-OXA-48	Forward: GCTTGATCGCCCTCGATT Reverse: GATTTGCTCCGTGGCCGAAA	56.0  59.4	238

TM: melting temperature of the primer.