Figure 2.

Isolation of CoaPLA₂ from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical C18 column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA₂ isolated from the venom of Crotalus Oreganus abyssus: Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).