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Effects of various reagents on fixation-induced blebbing of spread THP-1-derived macrophages. THP-1-derived macrophages were pre-fixed with or without wortmannin (100 nM for 1 h), U-73122 (10 μM for 30 min), LY294002 (50 μM for 30 min), or m-3M3FBS (100 μM for 2 min) followed by paraformaldehyde fixation. The number of cells measured in each group are indicated in Table 2 (∗p < 0.05, ∗∗∗p < 0.001 compared with the control).
