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. 2014 Mar 15;127(6):1318–1326. doi: 10.1242/jcs.143545

Fig. 3.

Fig. 3.

Eisosome defects in syj1 and its3 mutants. (A) Pil1 cortical filaments are dependent on Syj1 but not Inp53 for proper organization. Images are inverted maximum projections for Z-planes in the top half of the cell. Scale bar: 5 µm. (B) Eisosome organization is dependent on Its3. Cells were grown at 25°C and then switched to 32°C for 10 minutes. Images are inverted maximum projections from Z-planes in the top half of the cell. Scale bar, 5 µm. (C) Quantification of Pil1 cytoplasmic concentration in the indicated strain and temperature. Levels are presented as arbitrary fluorescence units (AFU) and represent mean ± s.d. for 25 cells.