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AZC treatment causes ROS formation. The strains were grown to exponential phase in SD medium before treatment with AZC (5 mM). Following incubation with DHR123, cell extracts were prepared and their fluorescence was measured (mean±s.d., n = 3). To assess the cell viability at each of the indicated timepoints, samples from each condition were adjusted to equal OD600 values and were serially diluted and spotted onto YEPD media.
