Fig. 3.

AZC treatment induces mitochondrial ROS formation. (A) The generation of ROS is abrogated in [rho⁰] strains. The indicated strains were grown to mid-exponential phase in SD medium, before treatment with AZC (5 mM) for 6 h. Following incubation with DHR123, cell extracts were prepared and their fluorescence was measured (mean±s.d., n = 3). (B) Transformation of the tsa1 mutant strain to [rho⁰] rescues its sensitivity to AZC but not to H₂O₂. The indicated strains were grown to stationary phase and spotted onto SD medium containing AZC (2 mM) or H₂O₂ (1 mM). (C) AZC toxicity is exacerbated under respiratory conditions. The indicated strains were grown to stationary phase and spotted onto SD medium (fermentative) or SGE medium (respiratory), with or without AZC (0.2 mM).