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. 2014 Mar 15;127(6):1327–1335. doi: 10.1242/jcs.144022

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Sites of protein aggregation are adjacent to active mitochondria. (A) Mitochondria undergo fragmentation following AZC treatment. A wild-type strain coexpressing Tsa1–GFP and mt–RFP was grown to mid-exponential phase before AZC (5 mM) treatment. Images were collected at 30 min and 2 h. (B) The sites of protein aggregation localise adjacent to mitochondria. The cells were fixed with formaldehyde and visualised on a Deltavision restoration microscope. A 3D projection was generated and snapshots of four angles up to 180° around the y-axis (0°, 60°, 120° and 180°) were processed. Four major Tsa1 granules are traceable through the angles, as indicated by the coloured arrows. (C) Mitochondrial membrane potential is maintained during AZC stress. Following AZC treatment, cells were incubated with DiOC₆(3). (D) No DiOC₆(3) staining was detected in a [rho⁰] mutant.