Fig. 1.

Growth of S. pombe is inhibited without cell death or G1 arrest following inhibition of TOR signalling by Torin1. (A) Wild-type cells grown on EMMG plates containing 25 µM Torin1, 300 ng/ml rapamycin or solvent. MeOH, methanol. (B-F) Liquid cultures were treated with 25 µM Torin1 or DMSO. (B) Cell number was measured and proliferation relative to vehicle calculated after 24 hours (C). (D) 500 cells were spread on YES plates and colony-forming units counted and shown relative to vehicle-treated cultures. (E) DNA content was analysed by flow cytometry. (F) Cell size was determined by forward-scatter flow cytometry.