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Immunofluorescence with antigen specific antibodies show the following: (a) normal staining for alpha dystroglycan in a control; (b) lack of staining for alpha dystroglycan (patient); (c) markedly reduced staining for Dysferlin; (d) moderately reduced staining for Merosin; (e) normal staining for beta dystroglycan and markedly reduced staining for (f) alpha sarcoglycan, (g) beta sarcoglycan, and (h) gamma sarcoglycan.
