Figure 3. Functional plasticity of sensory inputs to the olfactory bulb induced by olfactory learning.
(A and B) Intrinsic optical signal (IOS) imaging of activated glomeruli patterns evoked by different dilutions of Cin/Eu in different groups of mice (trained, naïve and exposed). For each odorant and group, all images for the different dilutions are from the same mouse. For the scale in (A) and (B), respectively, min ΔR/R (‰) = −2 and −2.5, max ΔR/R (‰) = 1.5 and 2. The magenta arrows highlight that more strongly activated glomeruli are visible in the trained mice at lower dilutions. (C) The average number of glomeruli activated by all odorants at higher dilutions (10−3 and 10−2 for Cin/Eu, 10−6 and 10−4 for IAA/EB) is significantly higher in the trained group (T., n = 5 mice; LSD test between T. and N. or E.: p<0.001) than in the naïve (N., n = 5 mice) or exposed (E., n = 5 mice; LSD test between E. and N. p=0.5) groups. (D and E) IOS imaging of the activated glomeruli patterns evoked by different dilutions of IAA/EB. (F) The average number of glomeruli activated by all odorants at lower dilutions (10−1 and 100 for Cin/Eu, 10−2 and 100 for IAA/EB) is similar for all groups (for all comparisons, LSD test p>0.1). For the scale in (D) and (E), respectively, min ΔR/R (‰) = −3.5 and −3, max ΔR/R (‰) = 3 and 2.5. Values are represented as mean ± SEM.