(a) The iron concentration in the liver, (b) iron concentration in the spleen, (c) zinc concentration in the liver, (d) zinc concentration in the spleen, and (e) copper concentration in the liver of control and curcumin-supplemented C57BL/6J mice. The concentrations of trace elements are expressed in mg per kg tissue (wet mass). Iron concentration was significantly lower in curcumin-supplemented mice in both the liver and the spleen. The concentrations of zinc and copper were not significantly regulated. Copper levels in the spleen were below the level of detection which was ~2 mg/kg. Values are expressed as means+SEM. ⁎P<0.05, ⁎⁎⁎P<0.001.