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. 2014 Feb 28;2:563–569. doi: 10.1016/j.redox.2014.01.018

Fig. 4.

Fig. 4

(a) Ferritin light (L) chain 1 mRNA levels in the liver of control and curcumin-supplemented C57BL/6J mice. Relative mRNA concentration was assessed using qRT-PCR and related to the average of two housekeeping genes (β-actin and GAPDH). Mean ferritin L concentration in the control group was set to be 1. The mRNA expression of ferritin L was significantly lower in curcumin-supplemented mice. Values are expressed as means+SEM. ⁎P<0.05. (b) Relative protein levels of ferritin L in the liver of control and curcumin-supplemented C57BL/6J mice as determined by Western blotting of the cytosolic fraction. Relative intensities of bands were quantified by densitometry and total lane protein was used as a loading control. Mean band intensity in the control group was set to be 1. Relative protein levels of ferritin L were significantly lower in curcumin-supplemented mice. Con, control group; Curc, curcumin group. Values are expressed as means+SEM. ⁎⁎⁎P<0.001.