Table 2.
Expression of tumour necrosis factor α (TNFα), interleukin 1β (IL1β), and 6 (IL6) mRNA in the liver and spleen; and of nuclear factor (erythroid-derived 2)-like 2 (NRF2), haem oxygenase 1 (HO1), NAD(P)H quinone oxidoreductase (NQO1), and γ-glutamyl cysteine synthetase (γ-GCS) heavy chain mRNA in the liver of control and curcumin supplemented C57BL/6J mice. Relative mRNA concentration was assessed using qRT-PCR and related to the average of two housekeeping genes (β-actin and GAPDH) in both tissues. The mean expression levels of genes in the control group were set to be 1. No significant differences between the groups were observed. Values are expressed as means±SEM.
| Control | Curcumin | ||
|---|---|---|---|
| Liver | TNFα | 1.00±0.14 | 1.11±0.07 |
| IL1β | 1.00±0.12 | 1.29±0.18 | |
| IL6 | 1.00±0.11 | 1.02±0.10 | |
| NRF2 | 1.00±0.09 | 1.12±0.08 | |
| HO1 | 1.00±0.12 | 1.17±0.10 | |
| NQO1 | 1.00±0.11 | 1.02±0.10 | |
| γ-GCS | 1.00±0.14 | 0.89±0.09 | |
| Spleen | TNFα | 1.00±0.06 | 1.16±0.09 |
| IL1β | 1.00±0.08 | 0.94±0.13 | |
| IL6 | 1.00±0.05 | 0.86±0.08 |