A, DNA sequencing results showing promoter methylation status in untreated LNCaP, PC3 and RWPE-1 cells. B, Representative figures showing methylation status in BPH, normal and tumor prostate tissues. * indicates individual CpG sites. For cell lines, a single primer set (BSP_F3 –R3) was used for BS-PCR whereas a nested PCR was performed for tissue samples using two primer sets BSP_F1 – R1 and BSP_F2 – R2.