Table 1.

Summary of the main features of the four HTS technologies.

	Roche 454	Illumina Genome Analyzer	ABI SOLiD	Helicos Heliscope
Sequencing method	Pyrosequencing	Reversible dye terminators	Sequencing by ligation	Single Molecule Sequencing
Read lengths	400 bases	100 bases	50 bases	35 bases
Sequencing run time	10 h	10 days	11-12 days	30 days
Total bases per run	500 Mb	20 Gb	100 Gb	35 Gb
Error Rate	0.1%	1.5%	4%	2-7%