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. 2010 Jul 1;1(2):143–165. doi: 10.3390/genes1010143

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© 2010 by the authors; licensee MDPI, Basel, Switzerland

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time versus number of loci assayed. The number of loci assayed has increased over time with the development of new technologies. Bisulfite sequencing (BS), methylation specific PCR (MSP) and combined bisulfite restriction analysis (COBRA) were introduced in the 1990s. These methods provided methylation data for up to a few hundred genes in a single experiment. Methylation arrays were introduced in the 2000s and provide methylation data for up to 100s of thousands of loci. (N.B. The latest oligonucleotide array available from Nimblegen allows for the interrogation of more than two million loci). Next-generation sequencing (NGS) was introduced in 2007 and provides single base pair resolution for billions of nucleotides.