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. 2010 Aug 18;1(2):244–262. doi: 10.3390/genes1020244

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© 2010 by the authors; licensee MDPI, Basel, Switzerland

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of EGFP in testis (A) and ovary (B) of double transgenic TSPY-Cre;Z/EG mice [16]. DAPI (blue) counterstained the DNA and EGFP fluorescence is shown in green. Within the testis (A), EGFP is exclusively expressed in round and elongated spermatids (stained in green), but not in other germ cells or somatic cells within the testis (stained blue). In B, an immunofluorescence image of an ovary from an 8-day old TSPY-Cre;Z/EG mice is shown. EGFP is expressed exclusively in maturing oocytes (stained green), while granulosa cells within the ovary were EGFP negative (stained blue). Figure 5 is published with the acknowledgement of Tatsuo Kido as the source.