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A) Cultured spirochetes from patient 3 tissue samples, 1000X darkfield microscopy, oil immersion. B) Cultured spirochetes in clumps from patient 4 tissue sample, heat fixed and stained with crystal violet, 1000X oil immersion. C) Borrelial spirochetes demonstrating fluorescence from tissue culture of patient 3, 1000X oil immersion, enlarged and cropped.
