Figure 2.

Nondenaturing PAGE-gel image (6%) of DNA assemblies with 5 μM total ss-DNA concentration (gel was prepared in 1× TAMg buffer (40 mM Tris base, 20 mM acetic acid, 7.5 mM MgCl₂·6H₂O), and run at 4 °C for 2 h at a 200 V potential). From left to right: lane 1 = HL5 DNA ladder, lane 2 = cyclic-[5′-C-3′]:[5′-C-3′]′ (normal annealing), lane 3 = cyclic-[5′-C-3′]:[5′-C-3′]′ (slow annealing), lane 4 = control-[5′-C-3′], lane 5 = cyclic-[5′-C-5′]:[5′-C-5′]′ (normal annealing), lane 6 = cyclic-[5′-C-5′]:[5′-C-5′]′ (slow annealing), lane 7 = control-[5′-C-5′], lane 8 = cyclic-[3′-C-3′]:[3′-C-3′]′ (normal annealing), lane 9 = cyclic-[3′-C-3′]:[3′-C-3′]′ (slow annealing), and lane 10 = control-[3′-C-3′].