Table 4. Melting Data for Cyclic Dimers, ff Dimers, and the Controls with 5 μM total DNA Concentration in TAMg Buffer (40 mM Tris Base, 20 mM Acetic Acid, 7.5 mM MgCl2·6H2O)a.
entry | short nameb | Tm (°C) | fwhm (°C) |
---|---|---|---|
1 | X:X′ | 49.7 ± 0.1 | 10.7 ± 0.1 |
2 | control-[5′-C-3′]c | 49.9 ± 0.2 | 8.9 ± 0.1 |
3 | control-[5′-C-5′] | 50.4 ± 0.1 | 8.7 ± 0.1 |
4 | control-[3′-C-3′] | 49.6 ± 0.1 | 8.9 ± 0.1 |
5 | control-[3′-T3CT3-5′] | 48.6 ± 0.2 | 10.0 ± 0.1 |
6 | cyclic-[5′-T3CT3-3′]:[5′-T3CT3-3′]′c | 52.6 ± 0.2 | 7.4 ± 0.1 |
7 | cyclic-[5′-T6-3′]:[5′-T6-3′]′ | 52.8 ± 0.2 | 6.7 ± 0.1 |
8 | cyclic-[5′-T6-5′]:[5′-T6-5′]′ | 53.0 ± 0.1 | 7.1 ± 0.1 |
9 | cyclic-[5′-T3-3′]:[5′-T3-3′]′ | 52.4 ± 0.1 | 7.6 ± 0.1 |
10 | ff-[3′-C-3′]:[5′-C-5′]′c | 62.9 ± 0.2 | 7.3 ± 0.1 |
11 | ff-[3′-C-3′]:[5′-T6-5′]′c | 58.2 ± 0.1 | 7.2 ± 0.1 |