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. 2014 Feb 5;118(9):2366–2376. doi: 10.1021/jp501041m

Table 4. Melting Data for Cyclic Dimers, ff Dimers, and the Controls with 5 μM total DNA Concentration in TAMg Buffer (40 mM Tris Base, 20 mM Acetic Acid, 7.5 mM MgCl2·6H2O)a.

entry short nameb Tm (°C) fwhm (°C)
1 X:X 49.7 ± 0.1 10.7 ± 0.1
2 control-[5′-C-3′]c 49.9 ± 0.2 8.9 ± 0.1
3 control-[5′-C-5′] 50.4 ± 0.1 8.7 ± 0.1
4 control-[3′-C-3′] 49.6 ± 0.1 8.9 ± 0.1
5 control-[3′-T3CT3-5′] 48.6 ± 0.2 10.0 ± 0.1
6 cyclic-[5′-T3CT3-3′]:[5′-T3CT3-3′]′c 52.6 ± 0.2 7.4 ± 0.1
7 cyclic-[5′-T6-3′]:[5′-T6-3′]′ 52.8 ± 0.2 6.7 ± 0.1
8 cyclic-[5′-T6-5′]:[5′-T6-5′]′ 53.0 ± 0.1 7.1 ± 0.1
9 cyclic-[5′-T3-3′]:[5′-T3-3′]′ 52.4 ± 0.1 7.6 ± 0.1
10 ff-[3′-C-3′]:[5′-C-5′]′c 62.9 ± 0.2 7.3 ± 0.1
11 ff-[3′-C-3′]:[5′-T6-5′]′c 58.2 ± 0.1 7.2 ± 0.1
a

For accurate comparison to ff dimers, only systems that afford cyclic dimers exclusively are listed.

b

For details, see Tables 1 and 2.

c

Melting profiles for these systems are shown in Figure 10.