Skip to main content
. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: J Biol Chem. 2006 May 5;281(28):19772–19780. doi: 10.1074/jbc.M601140200

Fig. 1. Native PAGE of different XSBD:E3 and XDD:E3 stoichiometries.

Fig. 1

E3 was incubated with stoichiometric amounts of XSBD (A) or XDD (B). As the amount of XSBD/XDD increases a shift from the position corresponding to E3 to that for the complex (←) is observed, indicating formation of a tight complex. At stoichiometries above 2:1 free XDD is seen in addition to complex, whereas free E3 is observed at stoichiometries below 2:1. Free XSBD migrates towards the cathode and out of the gel due to its high pI (9.9).