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. 2014 Mar 4;171(6):1408–1419. doi: 10.1111/bph.12410

Figure 5.

Figure 5

Effect of AGN 211335 and AGN 211336, and of the selective FAAH inhibitor URB597, on the hydrolysis of anandamide by intact RBL-2H3 cells. (A) The amounts (in cpm) of residual [14C]-anandamide or [14C]-ethanolamine formed from [14C]-anandamide hydrolysis by cells are shown. *P < 0.05 versus ‘no cells’; #P < 0.05 versus corresponding vehicle (DMSO, 0.1%) control, as assessed by anova followed by Bonferroni's test. −7, −6 and −5 denote the log of the concentrations used (M) for each compound. (B) The amounts, in pmol (mg of extracted lipids)-1 of anandamide, PEA and OEA in cells treated with vehicle (DMSO, 0.1%) or ionomycin (4 μM) with or without URB597 (1 μM) or AGN 211335 (2.5 μM). *P < 0.05 versus corresponding vehicle; #P < 0.05 versus corresponding ionomycin, as assessed by anova followed by Bonferroni's test. Incubations and cell processing were carried out as described in the Methods. Data are means ± SEM of n = 3 experiments.