Figure 6.

DAB immunostaining for the CB₁ receptor in the granular layer and the white matter areas of the cerebellum of SCA patients (C, C.I, D, D.I, F and F.I) and control subjects (A, A.I, B, B.I, E and E.I). The arrows indicate the CB₁ receptor-positive cells. The microphotograph shown in D.II corresponds to a detail of the CB₁ receptor-positive cells in the white matter of the folia of SCA patients (scale bars: A–F = 200 μm; A.I-F.I = 50 μm; D.II = 10 μm). The microphotographs of SCA cases correspond to subject #9 (SCA7) for the granular layer and the white matter of the folia, and to subject #10 (SCA6) for the white matter surrounding the dentate nucleus. The bar panel corresponds to the quantification of CB₁ receptor immunostaining in the three structures of SCA patients and control subjects. Values correspond to the mean density of labelling in a selected area and are expressed as means ± SEM of five subjects per group. Data were assessed by Student's t-test (*P < 0.05; **P < 0.01).