Figure 11.

Double-labelling immunofluorescence using antibodies for the CB₂ receptor and calbindin, and TOPRO-3 staining, in the Purkinje layer of the cerebellum of SCA patients (E–H and H.I) and control subjects (A–D, D.I), showing co-localization of CB₂ receptors and calbindin in SCA patients but not in control subjects (scale bars: A–H = 50 μm; D.I and H.I = 10 μm). The H.II panel corresponds to the orthogonal reconstruction from confocal z-series in x-z (below) and y-z (right) planes. This reconstruction confirms the co-localization of CB₂ receptor and calbindin in SCA patients (scale bars: H.II = 10 μm). The microphotographs of SCA samples correspond to subject #9 (SCA7).