Table 2.
List of antibodies used in immunohistochemical studies
| Antibody | Dilution (IHC) | Dilution (IF) | Class | Manufacturer |
|---|---|---|---|---|
| CB1 receptor | 1:300 | 1:100 | Polyclonal | Affinity BioReagents, Rockford, IL, USA |
| CB2 receptor | 1:100 | 1:50 | Monoclonal | R&D systems, Minneapolis, MN, USA |
| Calbindin D-28 K | 1:500 | 1:300 | Monoclonal | Sigma Chem., Madrid, Spain |
| Calbindin D-28 K | 1:250 | Polyclonal | Millipore, Billerica, MA, USA | |
| GFAP-Cy3 | 1:1500 | Monoclonal | Sigma Chem., Madrid, Spain | |
| GFAP | 1:500 | Monoclonal | AbD Serotec, Oxford, UK | |
| Iba-1 | 1:1000 | 1:1000 | Polyclonal | Wako, Osaka, Japan |
| Iba-1 | 1:75 | Polyclonal | Abcam, Cambridge, UK | |
| Cd68 | 1:50 | Monoclonal | Dako Cytomation, Glostrup, Denmark |
GFAP, glial fibrillary acidic protein; IF, immunofluorescence; IHC, immunohistochemistry.