Both TRPV1 and TRPA1 channels contribute to LE135-induced mechanical allodynia. Time course of mechanical allodynia in animals treated with LE135. Intraplantar injection of LE135 (30 nmol/10 μL) produced mechanical allodynia as reflected by a decrease in paw withdrawal threshold in Trpv1+/+ mice, which was not significantly reduced by i.p. injection of AMG9810 (10 mg·kg−1), or genetic deletion of TRPA1 or TRPV1 channels. However, LE135-elicited mechanical hypersensitivity was completely abolished in the Trpa1−/− mice pre-treated with AMG9810 (10 mg·kg−1). ***P < 0.001 versus LE135; +++P < 0.001 versus AMG9810; #P < 0.05, ##P < 0.01, ###P < 0.001 versus Trpv1−/−; and $$P < 0.01, $$$P < 0.001 versus Trpa1−/−.