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. 2014 Mar 4;171(6):1566–1579. doi: 10.1111/bph.12553

Figure 4.

Figure 4

Ligand-independent and aplaviroc-induced activation of [I116A]-CCR5. Constitutive activity normalized to Emax of the respective receptor in PI turnover and β-arrestin recruitment (A) and surface expression normalized to WT (B) of CCR5 WT (white columns) and [I11A]-CCR5 (black columns). (C and D) effect on PI turnover (C) and β-arrestin recruitment (D) of aplaviroc with CCL3 (white triangles) and without (black triangles) on [I116A]-CCR5. CCR5 WT basal level is indicated with dashed lines. Data is normalized to CCL3 Emax. COS-7 cells were used for PI turnover and N-terminally FLAG-tagged-based elisa, whereas U20S cells were used in β-arrestin recruitment. Statistical significance was calculated using Student's unpaired t-test. **P < 0.05, ***P < 0.001; ns, not significant; n = 3–25.