Figure 2.

X-band DEER of spin-labeled MMOB double cysteine mutants in H_ox–2B complex and the corresponding distance distributions. Left panels, background subtracted DEER data (black) and simulated fits (red) for spin-labeled MMOB double mutants L15C/N63C, L15C/D36C, G11C/N63C, and S5C/N63C in H_ox–2B complex. Right panels, the corresponding distance distributions derived from the DEER data (black) and from the crystal structure of H_ox–2B complex (red dashed lines). DEER data were processed by using DeerAnalysis 2011.¹⁶ The distance distributions derived from the crystal structure were modeled by using MMM program package 2011.2.¹⁷