Figure 3.

X-band DEER of spin-labeled MMOB double cysteine mutants in the H_red–2B complex and the corresponding core-to-tail distance distributions. Left panels, background subtracted DEER data (black) and simulated fits (red) for spin-labeled MMOB double mutants L15C/N63C, L15C/D36C, G11C/N63C, and S5C/N63C in the H_red–2B complex. Right panels, the corresponding distance distributions derived from the DEER data (blue); the DEER derived distance distributions of the corresponding H_ox–2B complex are also shown (black dashed lines). DEER data were processed by using DeerAnalysis 2011.¹⁶ The distance distributions derived from the crystal structure were modeled by using MMM program package 2011.2.¹⁷