Table 1. Summary of all SMM and FRAP analyses of YFP-GR, YFP-MR and YFP-GR deletion mutants.
| SMM | FRAP | |||||
| Plasmid | Treatment | Fraction | Fraction size (%) | D (µm2/s) | Fraction size (%) | Imm. time (s) |
| GR wt | Δ-Flu | Diffusing | 46.3±2.6 | 1.38±0.11 | 43±2.6 | - |
| Short | 53.7±2.6 | 0.05±0.004 | 33±2.1 | 0.8±0.1 | ||
| Long | 24±2.2 | 2.9±0.5 | ||||
| Dex | Diffusing | 55.1±2.0 | 1.31±0.13 | 44±2.2 | - | |
| Short | 44.9±2.0 | 0.03±0.009 | 33±2.6 | 0.7±0.1 | ||
| Long | 23±2.6 | 2.3±0.3 | ||||
| Predn | Diffusing | 60.7±3.1 | 2.20±0.11 | 42±2.5 | - | |
| Short | 39.3±3.1 | 0.09±0.008 | 36±1.6 | 0.7±0.1 | ||
| Long | 22±2.5 | 4.0±0.8 | ||||
| Csol | Diffusing | 55.6±3.5 | 1.77±0.10 | 58±2.0 | - | |
| Short | 44.4±3.5 | 0.04±0.003 | 19±3.8 | 0.5±0.1 | ||
| Long | 23±2.6 | 2.0±0.0 | ||||
| Cort | Diffusing | 74.1±3.3 | 2.49±0.24 | 66±2.2 | - | |
| Short | 25.9±3.3 | 0.08±0.024 | 26±3.7 | 0.6±0.1 | ||
| Long | 8±2.5 | 1.2±0.3 | ||||
| RU486 | Diffusing | 69.1±2.4 | 2.86±0.11 | 66±1.6 | - | |
| Short | 30.9±2.4 | 0.14±0.018 | 24±3.1 | 0.5±0.1 | ||
| Long | 10±2.6 | 1.4±0.3 | ||||
| MR wt | Aldo | Diffusing | 54.1±3.4 | 1.43±0.04 | 45±1.7 | - |
| Short | 45.9±3.4 | 0.05±0.002 | 32±2.0 | 0.8±0.1 | ||
| Long | 23±2.1 | 2.9±0.5 | ||||
| Cort | Diffusing | 50.7±1.4 | 1.37±0.13 | 47±2.1 | - | |
| Short | 49.3±1.4 | 0.08±0.005 | 31±2.3 | 0.7±0.1 | ||
| Long | 22±2.5 | 3.4±0.8 | ||||
| Csol | Diffusing | 51.5±0.8 | 1.96±0.19 | 44±2.2 | - | |
| Short | 48.5±0.8 | 0.05±0.002 | 32±2.9 | 0.6±0.1 | ||
| Long | 24±2.7 | 2.3±0.3 | ||||
| DOC | Diffusing | 60.5± 3.6 | 1.60±0.13 | 57±1.5 | - | |
| Short | 39.5±3.6 | 0.06±0.003 | 23±3.0 | 0.6±0.1 | ||
| Long | 20±3.1 | 2.3±0.3 | ||||
| Dex | Diffusing | 64.3±6.0 | 1.74±0.20 | 67±2.1 | - | |
| Short | 35.7± 6.0 | 0.04±0.003 | 22±4.4 | 0.7±0.1 | ||
| Long | 11±3.1 | 1.7±0.5 | ||||
| Spiro | Diffusing | 78.8±2.3 | 2.71±0.05 | 71±3.5 | - | |
| Short | 21.2±2.3 | 0.06±0.018 | 23±3.7 | 0.5±0.1 | ||
| Long | 6±1.6 | 1.2±0.3 | ||||
| Epler | Diffusing | 68.2±6.6 | 2.49±0.12 | 66±1.6 | - | |
| Short | 31.8±6.6 | 0.06±0.004 | 25±2.7 | 0.6±0.1 | ||
| Long | 9±2.3 | 1.7±0.5 | ||||
| GR ΔAF-1 | Dex | Diffusing | 46.5±1.9 | 0.61±0.08 | 57±3.0 | - |
| Short | 53.5±1.9 | 0.00±0.006 | 18±3.6 | 0.6±0.1 | ||
| Long | 25±3.4 | 2.1±0.4 | ||||
| Cort | Diffusing | 64.7±2.8 | 2.69±0.08 | 62±2.5 | - | |
| Short | 35.3±2.8 | 0.05±0.012 | 27±4.0 | 0.6±0.1 | ||
| Long | 11±2.3 | 1.6±0.3 | ||||
| GR ΔDBD | Dex | Diffusing | 75.6±3.4 | 2.27±0.15 | 66±1.6 | - |
| Short | 24.4±3.4 | 0.01±0.006 | 25±2.7 | 0.5±0.1 | ||
| Long | 9±2.3 | 1.4±0.3 | ||||
| Cort | Diffusing | 81.3±1.0 | 2.37±0.19 | 79±3.1 | - | |
| Short | 18.7±1.0 | 0.06±0.004 | 18±3.9 | 0.5±0.1 | ||
| Long | 3±1.5 | 0.6±0.3 | ||||
| GR ΔLBD | Dex | Diffusing | 86.5±1.9 | 2.71±0.08 | 82±3.3 | - |
| Short | 13.5±1.9 | 0.03±0.010 | 16±3.1 | 0.4±0.1 | ||
| Long | 2±1.3 | 0.4±0.3 | ||||
Short, ‘short’ bound fraction; long, ‘long’ bound fraction; D, diffusion coefficient; imm. time, average immobilization time; Δ-Flu, Δ-Fludrocortisone; dex, dexamethasone; Predn, prednisolone; csol, cortisol; cort, corticosterone; aldo, aldosterone; DOC, deoxycorticosterone; spiro, spironolactone; epler, eplerenone. Fraction size and diffusion coefficient for immobile fraction in SMM are for both immobile fractions combined. Results are represented as best fit ± SEM (of three separate fits) for SMM and as average ± SEM of top 10% fits for FRAP.