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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Oncogene. 2011 Jun 6;31(1):13–26. doi: 10.1038/onc.2011.211

Figure 1. Daxx-dependent stability of cyclin B.

Figure 1

A) Western blot analysis of Daxx depletion in HEp2 cells. B) Control- and Daxx-depleted HEp2 cells were synchronized by a double thymidine block (0 hr) and then released into normal media for progression through mitosis (6–11 hrs). Top: Western blot analysis of cyclin B protein stability. Bottom: Relative quantization of cyclin B protein levels (normalized to actin). Cyclin B protein is stabilized longer in Daxx-depleted cells (at 9.5–11 hrs, post-thymidine release), indicating that Daxx-depleted cells are delayed in mitosis. Data show a representative experiment out of four.