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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Exp Biol Med (Maywood). 2012 Aug 2;237(7):832–844. doi: 10.1258/ebm.2012.012028

Figure 4. Anti-proliferative properties of ABC294640.

Figure 4

(A) MDA-MB-468 cells treated with vehicle or ABC394640 for 48 h were fixed using 3.7% formaldehyde in PBS, permeabilized using cold methanol and incubated with anti-Ki-67 antibody (red). Cells were washed (with PBS) and DAPI (blue) nuclear stained before imaging. Three independent experiments were performed with representative pictures shown here. (B) Quantitation of Ki-67 staining was determined as a percentage of total positive cells/image. The vehicle control was set to 1 for comparison with ABC294640 treatment.