Table 1. General Genome sequencinga features and annotationb features of L. rhamnosus clinical isolates and L. rhamnosus GG and L. rhamnosus Lc705 genomic features.
Organism | Total Reads | genome coverage | Number of contigsc (>200 bp) | Genome size (Mbp) | Coding sequences (CDS) | rRNA operons | tRNA genes | GC content (%) | Hypothetical proteins (total) |
LRHMDP2d | 77,329 | 17 X | 51 | 2.91 | 2,910 | 7 | 56 | 46.6 | 815 |
LRHMDP3 d | 75,985 | 17 X | 47 | 2.91 | 2,925 | 6 | 57 | 46.6 | 819 |
GGe | - | - | - | 3.01 | 2944 | 5 | 57 | 47 | 734 |
Lc705e | - | - | - | 3.03 | 2992 | 5 | 61 | 47 | 760 |
Using Roche GS(FLX+) pyrosequencing.
Based on results merged from the RAST (Rapid Annotation using Subsystem Technology) server and tRNAscan-SE.
assembled by Newbler Assembler 2.6.
Both L.rhamnosus clinical isolates (LRHMDP2 and LRHMDP3) were sourced from infected dental pulps from carious teeth categorised as representing the initial stages of infection of pulp tissue.
[13].